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Molecular characterization and zoonotic potential of Giardia intestinalis populations from pets.
Hammerbauerová, Iva ; Tůmová, Pavla (advisor) ; Votýpka, Jan (referee)
Giardia intestinalis is a single-celled intestinal parasite infecting humans and animals. The species is divided into eight genetic groups, assemblages, with different host specificity. Stool samples from 99 dogs, 61 cats and 22 chinchillas were examined for the presence of Giardia using microscopy and PCR diagnostics. The found populations were assigned to assemblages using a multi-locus genotyping scheme, with the goal of mapping the occurrence of zoonotic assemblages A and B and evaluating the risk of transmission of Giardia from pets to humans. The Giardia prevalence in examined dogs was 36,4%. The majority of dog infections was caused by dog-specific assemblages D and C. Individual cases of infection with assemblage F, or a mix of assemblages A+D, A+F, B+D, C+D and D+F were also detected. The prevalence in cats was 14.8%, and the dog assemblages C and D prevailed as well. In individual cases, cats were infected with assemblages A or F, which is specific for cats. The highest prevalence, 85.7%, was detected in chinchillas. The majority of chinchilla infections was caused by the zoonotic assemblage B (88.9%). The found sequences were compared to those obtained from animals with clinical giardiasis, but no identical matches were found between these two pools. The nature of mixed infections was studied by...
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Fossil associations of the Dobrotivá Formation
Luptáková, Monika ; Kraft, Petr (advisor) ; Mergl, Michal (referee)
The bachelor thesis is focused on the overview about a fundamental knowledge on the fossil associations of the Dobrotivá Formation. This unit represents a distinct level of the Prague Basin volcano-sedimentary infill formed during the boundary interval of the Middle and Upper Ordovician. Its main part is composed of the acquired and published data in the wider context of the neighboring paleocontinents and areas of peri-Gondwana. The thesis briefly describes the development of the Prague Basin and its paleogeography. It summarizes the lithology of the Dobrotivá Formation, its stratigraphic classification and the recently published knowledge on faunal associations in the Dobrotivá Formation, including their paleobiogeography and paleoecology. The bachelor thesis is considered to be a basis for further study of the Dobrotivá Formation. Key words: Ordovician, Prague Basin, assemblages, localities, stratigraphy, paleobiogeography
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Testate amoebae assemblages (Protozoa, Rhizopoda) from the National Nature Reserve Žofínský prales )Novohradské hory Mts., Czech Republic)
Balík, Vladimír
The soil and moss testate amoebae fauna of the National Nature Reserve Žofínský prales in the Novohradské hory Mts. was investigated in the years 1988 and 1997-1999. In total 197 testate amoebae species, varieties and forms were determined here. National Nature Reserve Žofínský prales belong from faunistical point of view to the richest areas in the Czech Republic. On the basis of coenological analysis of the primary data set and on the basis of application of the cluster and ordination analyses were determined here four basic testate amoebae assemblages: beech forest assemblage, acidophilous spruce forest assemblage, wet spruce forest assemblage and assemblage of moss carpets on soil, fallen trunks and big stones. In these four basic testate amoebae assemblages was studied the influence of microhabitats characterised by different plant undergrowth and different microclimatic conditions on qualitative and quantitative parameters of these testate amoebae assemblages. No significant impact of these microhabitats was determined.
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